A novel plasmid addiction system for large-scale production of cyanophycin in Escherichia coli using mineral salts medium

摘要

Introduction Hitherto the production of the biopolymer cyanophycin (CGP) using recombinant Escherichia coli strains and cheap mineral salts medium yielded only trace amounts of CGP (<0.5%, w/w) of the cell dry matter (CDM). This was probably due to the instability of the plasmids encoding the cyanophycin synthetase.