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Survival of embryos and calves derived from somatic cell nuclear transfer in cattle: a nationwide survey in Japan

机译:牛体细胞核移植产生的胚胎和小牛的存活:日本的全国性调查

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摘要

To obtain data concerning the survival of embryos and calves derived from somatic cell nuclear transfer (SCNT) in Japan, a nationwide survey was carried out in April, 2009. As a result, data concerning 3264 embryo transfers (ETs) with SCNT embryos which produced 301 calves were accumulated and their survival was analyzed. The present survey revealed that survival rates of transferred bovine embryos and produced calves derived from SCNT had not improved over a decade (1998–2007). A remarkable feature of the pregnancies with SCNT embryos was a high incidence of spontaneous abortions. When the decade was divided by the occurrence of bovine spongiform encephalopathy (BSE) in 2001, significant decreases in the ‘after BSE’ period (2002–2007) were observed in the percentages of calves born (P?
机译:为了获得有关日本的源自体细胞核移植(SCNT)的胚胎和犊牛存活的数据,2009年4月进行了全国范围的调查。结果,涉及3264个SCNT胚胎的胚胎移植(ET)的数据积累了301只犊牛,并对其存活进行了分析。本次调查显示,十年来(1998-2007年),转移的牛胚胎和源自SCNT的牛犊的存活率没有提高。 SCNT胚胎妊娠的显着特征是自然流产的发生率很高。当2001年用牛海绵状脑病(BSE)的发生除以这十年时,观察到“出生后的牛犊”(2002-2007年)的犊牛百分比(P≤0.01)显着下降。出生时(P 0.05),犊牛活24小时(P 0.05)和6个月(P 0.01)。在“疯牛病之后”期间,在ET发生后61-99天内发生的流产显着增加(P 0.01)。作为BSE检查的一部分,在BSE检查中,在10–15°C的牛卵巢存储15–20?h时,卵母细胞中发生的某些种类的再生可能会对SCNT胚胎产生一些负面影响。 。

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