CONFIGURATIONS OF A FLOW INJECTION SYSTEM FOR PERFUSION STUDIES OF ADHERENT CELLS

摘要

This paper describes three configurations of a now injection apparatus designed to be the fluidic drive for perfusion studies of cultured adherent cells. The apparatus was coupled to a now-through perfusion chamber that was specifically designed for live cell perfusion using fluorescence microscopy as the detector. The instrument consists of two linear syringe pumps and a multiposition selector valve which, under computer control, allowed sequential injection, fluid switching, and now injection to be performed with minimal system reconfiguration. When the apparatus is coupled to a dual inlet perfusion chamber, target cells can be exposed to very steep reagent pulses, while the traditional single inlet perfusion chamber allows more flexibility and provides a more gradual increase in reagent concentration, The most significant salient feature of the system is the ability to generate very steep pulses-a desirable feature for cell perfusion studies.