STATIONARY PHASES

摘要

In biotechnological production of protein Pharmaceuticals, analytical chemists must verify the correct structure of the recombinant products with respect to primary structure (amino acid sequence), three-dimensional structure (including correct -S-S- bonds), post-translational modifications, aggregation behavior, and immunological and biological activity. They also must detect and quantify any degradation products. In a typical characterization scheme the recombinant product must be separated and isolated, and most often an enzymatic degradation is performed. The fragments are separated, isolated, and characterized by amino acid sequence, mass, and amino acid analysis. All these procedures depend on some sort of LC, which is probably the most common individual tool in today's protein chemistry laboratory.