Reagentless tyrosinase enzyme electrodes: effects of enzyme loading, electrolyte pH, ionic strength, and temperature

摘要

We have prepared a reagentless enzyme cativity sensor based on the mediated reduction of oxygen by tyrosinase coimmobilized in an osmium redox polymer hydrogel on glassy carbon electrode surfaces. The activity of this sensor is shown to be influenced by the enzyme loading, yielding an optimum activity for 41.7/100(w/w) enzyme in the deposition solution.