Solvent Microextraction as a Speciation Tool: Determination of Free progesterone in a Protein Solution

摘要

A 1-μL drop of n-octane suspended from the tip of a microsyringe needle in 500mL of stirred aqueous solu- tion is used to extract unbound progesterone in the presence of 1/100(w/v) bovine serum albumin (BSA) for analysis by gas chromatography (GC). The observed extraction kinetics are in good agreement with a proposed convective-diffusive mass transfer model which accounts for diffusion of both free and bound species in the aqueous Nernst diffusion film adjacent to the interface.