Peptide Separation in Normal Phase Liquid Chromatography

摘要

A new method is established for separating peptides in normal phase liquid chromatography using TSK gel Amide-80, carbamoyl groups bonded to a silica gel matrix, and an acetonitrile-water solution containing 0.1/100 tri- fluoroacetic acid. Peptide retention time increased with acetonitrile concentration in the initial eluent. Hydro- philic peptides with no retention in a reversed phase column were retained and separated in the present method.