Open-Tubular Capillary Electrochromatography with an On-Line Ion Trap Storage/Reflectron Time-of-Flight Mass Detector for Ultrafast Peptide Mixture Analysis

摘要

In this work, a novel open-tubular column(OTC) capillary electrochromatography(CEC) system has been coupled to an on-line ion trap storage/reflectron time-of-flight mass spectrometer for ultrafast peptide mixture analysis. Re- versed-phase OTCs prepared by the sol-gel process were coated with an amine, which greatly enhanced the elec- troosmotic flow in an acidic buffer solution and consider- ably reduced nonspecific adsorption between the peptides and the column wall.