The rapid measurement of ABO blood type by using surface-plasmon resonance sensor

摘要

A surface plasmon resonance(SPR) sensor is an instrument that can respond to an immunoreaction. The sensor chip of SPR was prepared by immobilizing a monoclonal antibody (anti-A or B) on a gold thin layer for inspection of the ABO blood type. When a phosphate-buffered saline solution (pH 7.2) containing saliva or bloodstain was flowed into the cell, the following reactions were observed for each sample: 1) The A-type sam- ple responded to the sensor-chip immobilizing anti-A monoclonal antibody (anti-A chip), but did not respond to the anti-B chip. On the other hand, 2) the B-type responded to the anti-B chip, but did not respond to the anti-A chip. 3) The O-type sample did not respond to either the anti-A chip or anti-B chip. 4) The AB-type sample responded to both the anti-A and anti-B chips. Thus, this method was rapid, sensitive and simple for inspecting the ABO blood type for saliva and blood samples.