キャピラリーゾーン電気泳動法による18-クラウン-6とフェニルアルキルアンモニウムの錯形成反応の解析

摘要

Some complexing agents and surfactants sensitize luminol chemiluminescence (CL) with Fe(III) or Fe(II) as a catalyst; also the effect of the sensitizing reagents on the CL intensity was examined in sequential injection analysis (SIA). Solutions of luminol, H_2O_2 and a sample solution containing Fe(III) or Fe(II) were sequentially introduced into a holding coil, and the zones of the sucked solutions were immediately propelled to a CL detection cell; the CL intensity was measured. In the case of the complexing agents as additives, tartrate and oxalate ions in the sample solution were most effective to improve the CL intensity. When surfactants were used as additives, the chemiluminescence was sensitized over the critical micelle concentrations of cat-ionic cetyltrimethylammonium bromide and anionic sodium dodecylsulfate. Sensitization with the complexing agents and the surfactants in SIA are much more effective than in flow injection analysis. Since mixing of the sucked zones is not sufficient in SIA, the chemiluminescence would have been effectively sensitized.%キヤピラリーゾーン電気泳動法（capillary zone electro-phoresis，CZE）は，溶質の電気泳動移動度の差異に基づく分離分析法であり，内径50～100μm程度の細いキヤピラリーを用いることで高い分離能が得られる利点が注目され，小さなイオンからタンパク質等の大きな分子の分離分析に広く普及している.C甲こおける分析対象物質の分離改善方法として，各種モディファイヤーが有効に利用されている．18-クラウンー6（18C6）は，アンモニウム基と複数の水素結合を形成することから，各種アミンの分離分析に使用される．これまでに，CZEにおいて，有機アミン，神経伝達物質，シスートランス異性僻）等の分離改善に用いられてきた．