Imaging MS Methodology for More Chemical Information in Less Data Acquisition Time Utilizing a Hybrid Linear Ion Trap−Orbitrap Mass Spectrometer

摘要

A novel mass spectrometric imaging method is developednto reduce the data acquisition time and provide richnchemical information using a hybrid linear ion traporbitrapnmass spectrometer. In this method, the linearnion trap and orbitrap are used in tandem to reduce thenacquisition time by incorporating multiple linear ion trapnscans during an orbitrap scan utilizing a spiral raster stepnplate movement. The data acquisition time was decreasednby 43-49% in the current experiment compared to thatnof orbitrap-only scans; however, 75% or more time couldnbe saved for higher mass resolution and with a highernrepetition rate laser. Using this approach, a high spatialnresolution of 10 μm was maintained at ion trap imaging,nwhile orbitrap spectra were acquired at a lower spatialnresolution, 20-40 μm, all with far less data acquisitionntime. Furthermore, various MS imaging methods werendeveloped by interspersing MS/MS and MSn ion trapnscans during orbitrap scans to provide more analyticalninformation on the sample. This method was appliednto differentiate and localize structural isomers ofnseveral flavonol glycosides from an Arabidopsis flowernpetal in which MS/MS, MSn, ion trap, and orbitrapnimages were all acquired in a single data acquisition.