Sensitive DNA-Based Electrochemical Strategy for Trace Bleomycin Detection

摘要

Bleomycins (BLMs) are widely used in combination withnchemotherapy for the treatment of a variety of cancers.nThe clinical application of BLMs is featured by thenoccurrence of sometimes fatal side effects, such as renalnand lung toxicity, and the potential dose-limiting sideneffect of pulmonary fibrosis. Therefore, it is highly desirablento develop a sensitive method to quantitativelyndetermine the BLM content in both pharmaceuticalnanalysis and clinical samples, to make full use of therapeuticnefficacy and to weaken its toxicity. Here, wenproposed a simple, rapid, and convenient electrochemicalnassay for trace BLM detection. A reported DNA motif, asnsubstrate for BLMs, is prepared to self-assemble onto thengold electrode to fabricate an electrochemical DNA (EDNA)nsensor, with a terminus tethered on the electrodensurface and the other terminus labeled with ferrocenylnmoiety as a signal reporter to form a stem-loop structure,ngiving an arise of remarkable faradaic current. In thenpresence of Fe(II) ·BLM, the E-DNA sensor undergoes thenirreversible cleavage event, which can be transduced intona significant decrease in current peak. This proposednsensor reveals an impressive sensitivity as low as 100 pMnBLMs and exhibits a good performance as well as innserum sample. Considering the high sensitivity andnspecificity of this proposed sensor, as well as the costeffectivenand simple-to-implement features of the electrochemicalntechnique, we believe that this method showsndistinct advantages over conventional methods and it isna promising alternative for the determination of tracenamounts of BLMs in clinical samples.