Nanoparticle-Based Biocompatible and Long-Life Marker for Lysosome Labeling and Tracking

摘要

In this paper, a novel biocompatible and long-life lysosomenlabeling and tracking method based on dye entrappednsilica nanoparticles (DSiNPs) has been putnforward. Through colocalization studies using LysoTrackernGreen as the standard lysosome marker, it has beenndemonstrated that DSiNPs selectively accumulated innlysosomes of Hela cells and the photostability of DSiNPsnassociated with lysosomes was detectable, at least, 30ntimes as long as that of LysoTracker Green involved innlysosomes. By comparison with LysoTracker Green andnAlexa 488-dextran, the fluorescence of DSiNPs could bendetected over a 5-day postrecultivation period and thenstaining pattern in lysosomes could be well retained afterncell fixation and permeabilization. In addition, resultsnfrom MTT assays showed that DSiNPs did not affect thenviability of Hela cells at the concentration for lysosomenlabeling. Primary applications of DSiNPs were thennfurther performed in lysosome tracking in chloroquinetreatednHela cells, and lysosome labeling of differnet cellnlines, including MCF-7 cells, MEAR cells, and MSC cells.nThese results indicated that DSiNPs, therefore, can benused as a biocompatible, long-life, and highly photostablenlysosome marker for lysosome-related studies.