Specific Nucleic Acid Detection Using Photophysical Properties of Quantum Dot Probes

摘要

In the present study, we report a novel separation-freenmethod to detect and quantify avian influenza virus An(H5N1) nucleic acid without amplification, based on thenalteration of photophysical parameters of quantum dotn(QD) probes after hybridization with specific complementaryntarget DNA. The target DNA was quantified in ancustom-made portable device by simultaneously measuringnlifetime and quenching of the QD probes. QD probesn(25-mer) showed a 30% lifetime reduction and 40%nfluorescence quenching when hybridized with complementaryn25-mer target DNA. In comparison with a conventionalnQD-based assay, this assay provides a simplenquantitation of nucleic acids with a single labeling step.