Laser Diode Thermal Desorption/Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry Analysis of Selected Steroid Hormones in Wastewater: Method Optimization and Application

摘要

A rapid and reliable method enabling high-throughputnsample analysis for quicker data generation, detection,nand monitoring of eight selected steroid hormones innwater matrixes was developed and validated. Our ap-nproach is based on a novel sample introduction method,nthe laser diode thermal desorption/atmospheric pressurenchemical ionization (LDTD/APCI) coupled to tandemnmass spectrometry (MS/MS). The optimization of instru-nmental parameters and a method application are pre-nsented. Our method was successfully applied to spikedneffluent wastewater in the low-nanogram per liter concen-ntrations with total analysis time reduced to seconds (15ns) using LDTD/APCI-MS/MS compared to minutes withntraditional liquid-chromatography coupled to tandemnmass spectrometry (LC MS/MS) following solid-phasenextraction (SPE). The instrumental detection limits fornLDTD/APCI-MS/MS ranged from 5 to 24 µgL 1 andnfrom 13 to 43 ng L 1 for the method detection limits.nCalibration curves in wastewater matrix showed goodnlinearity (R2 0.99), and precision (intraday andninterday) was below 20%. This work demonstrates thatnLDTD/APCI-MS/MS could be used for fast and effec-ntive quantitative analysis of emerging contaminants inndifferent water matrixes with reduced cost by eliminat-ning the chromatography step used in traditionalnLC MS/MS.