Rapid determinations of saccharides in high-energy drinks by short-capillary electrophoresis with contactless conductivity detection

摘要

The methodology for separations of saccharides in standard electrophoretic systems has been transferred to the short-capillary electrophoresis format. The laboratory-designed apparatus used employs a quartz capillary with an internal diameter of 10 μm, a total length of 10 cm, and an effective length of 4 cm, in combination with contactless conductivity detection. It has been applied to separations of neutral mono- and disaccharides. The saccharides are separated in the anionic form, in solutions of alkali hydroxides, namely, KOH, NaOH, and LiOH. The separation of a model mixture of five saccharides (sucrose, lactose, glucose, fructose, and ribose) takes less than 1 min, the LOD equaling 15, 35, 19, 17, and 24 mg L−1 and the LOQ equaling 52, 117, 63, 53, and 79 mg L−1 for sucrose, lactose, glucose, fructose, and ribose, respectively. The technique developed has been used to determine sucrose, glucose and fructose in high-energy drinks. The separation is finished within less than 50 s; the saccharide contents determined are identical with the declared values within the reliability interval in most cases, the RSD value being mostly less than 2 %. In general, the separation system developed is very convenient for rapid analyses of large sets of similar samples, e.g., in product quality control or environmental monitoring.