Raid prediction of rifampin susceptibility of mycobacterium tuberculosis

摘要

ew evaluated the relationship between rifampin (RIF) susceptibility and amino acid substitution in rpoB gene of Mycobacterium tuberculosis and the usefulness of rpoB gene sequencing in the rapid pre- diction of RIF susceptibility of M. Tuberculosis in clinical specimens. A total of 76 genetic alterations in the 69 bp core region of rpoB gene were detected in 74 of 130 M. Tuberculosis strains. Examination of the correlation between the minimum inhibitory concentrations (MICs) of FIF and amino acid substi- tutions in the 69 bp core region of rpoB gene revealed that all 43 strains containing amino acid sub- stitution with Leu or Trp in codon 531 showed RIF-resistant phenotypes, with MICs≥64 μg/ml.