The Requirement of Intercellular Adhesion Molecule-1 for Neutrophil Respiratory Burst in the Pulmonary Circulation of Rats Infused with Endotoxin

摘要

Recently, we demonstrated direct evidence of respiratory burst of the neutrophil in the pulmonary circulation of the endotoxin-infused rat(Am. J. Respir. Crit. Care med. 1995; 152:348-354). To deter- mine the role of intercellular adhesion molecule-1(ICAM-1) in this model, we neutralized ICAM-1 us- ing antirat ICAM-1 monoclonal antibody(mAb) 1A29. We observed and measured the number of sticking leukocytes and the amount of hydrogen peroxide(H_2O_2) in the pulmonary circulation of the endotoxin-infused rat by means of a fluoro-imaging technique.