Influence of Agrobacterium Strain, Culture Medium, and Cultivar on the Transformation Efficiency of Vitis vinifera L.

摘要

Like most woody plants, grapevine is classically improved through vegetative methods or conventional breeding. Despite significant progress in genetic engineering, some cultivars of Vitis vinifera are recalcitrant to transformation. This study investigated factors involved in the response of V. vinifera to transformation by Agrobacterium. Embryogenic cultures of several V. vinifera cultivars (Portan, Danuta, Shiraz, and Chardonnay) were established on four solid media (GS1CA, C1P, C1+P, and C1A100 differing in mineral Compounds, plant growth regulator type and level, and gelling agent.