Encapsulation of Nanoparticles Using Nitrilotriacetic Acid End-Functionalized Polystyrenes and Their Application for the Separation of Proteins

摘要

The use of nitrilotriacetic acid end-functionalized polystyrenes (NTA-PS) as a multifunctional nanocarrier for the aqueous dispersion of CdSe, γ-Fe_2O_3 and gold nanoparticles (NPs) is described. When the amphiphilic end-functionalized polystyrenes and NPs are dissolved together in tetrahydro-furan, the addition of water causes the spontaneous formation of micellar aggregates, resulting in the successful encapsulation and aqueous dispersion of NPs. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), photoluminescence (PL) spectroscopy, and vibrating sample magnetometer (VSM) are used to characterize the structure and properties of the NPs-containing micellar aggregates (nanocarrier). After complexation of Ni_(2+) with NTA on the surface of the nanocarrier containing γ-Fe_2O_3, specific binding between Ni-NTA complex and histidine-tagged (His-tagged) proteins enables selective separation of His-tagged proteins using a magnet.