On-Chip Fabrication of Paclitaxel-Loaded Chitosan Nanoparticles for Cancer Therapeutics

摘要

The use of solvent-free microfluidics to fine-tune the physical and chemical properties of chitosan nanopartides for drug delivery is demonstrated. Nanoparticle self-assembly is driven by pH changes in a water environment, which increases biocompatibility by avoiding organic solvent contamination common with traditional techniques. Controlling the time of mixing (2.5-75 ms) during nanoparticle self-assembly enables us to adjust nanoparticle size and surface potential in order to maximize cellular uptake, which in turn dramatically increases drug effectiveness. The compact nanostructure of these nanopartides preserves drug potency better than previous nanopartides, and is more stable during long-term circulation at physiological pH. However, when the nanopartides encounter a tumor cell and the associated drop in pH, the drug contents are released. Moreover, the loading efficiency of hydrophobic drugs into the nanopartides increases significantly from previous work to over 95%. The microfluidic techniques used here have applications not just for drug-carrying nanoparticle fabrication, but also for the better control of virtually any self-assembly process.