Dynamic digital fluorescence ratio imaging of cell calcium in vascular endothelial cells

摘要

AIM: To study the spatial and temporal distribution of intracellular Ca~2+ concentration in cultured bovine pulmonary artery endothelial (BPAE) cells. METHODS: Cultured BPAE cells were loaded with Fura-2 and observed under an inverted microscope Coupled to a microfluorimeter, which enables pixel-to- Pixel ratio imaging of the BPAE cells in real time. CONCLUSION: Changes of cell Ca~2+ Upon stimulation by various agents that ated at Different intracellular sites were found to be temporarily And spatially heterogeneous among BPAE cells. At the Single cell level, Ca~2+ elevation seemed to occur Initially near the peripheral region followed by the Nuclear region.