THE CYTOTOXIC EFFECTS OF CRUDE BILE ON HUMAN PANCREATIC CANCER CELL LINES

摘要

Objective To identify effects of bile acids on pancreatic cancer. The ultrastructure and growth of PANC-1 and MIA PaCa-2 cell lines in crude bile modified medium were studied. Methods The growth of PANC-1 and MIA PaCa-2 cells in RPMI 1640 with or without 1/100, 2/100 and 4/100 of the purified crude bile (containing total bile Acids 10.17 mol/L) was assessed for 2, 4, 6, 8d by using MTT assay to determine inhibitory rate.