Dual modality live cell imaging with multiple-wavelength digital holography and epi-fluorescence

摘要

We apply multiple-wavelength digital holography in combination with epi-fluorescence microscopy in order to generate quantitative phase and fluorescence information from cell samples. While digital holography provides high precision morphological information, the addition of fluorescence supplies the specificity needed to identify cellular constituents. By the application of multiple-wavelength digital holography it is possible to obtain the complete wavefront data deterministically and in real-time. We demonstrate this dual-mode imaging capability through the investigation of living cells.