FTY720 ameliorates GvHD by blocking T lymphocyte migration to target organs and by skin fibrosis inhibition

摘要

FTY720 suppressed Alloreactive T-cell responses in mice and human with reducing pro-inflammatory cytokines. , In the mixed lymphocyte reaction assay, a total of 10 B/c(B6) splenic T cells (responders) were incubated with 10 irradiated B/c(B6) (syngeneic stimulators [Syn]) or B6(B/c) (allogeneic stimulators [Allo]) splenic APCs for 4 days. In the mixed lymphocyte reaction assay, human PBMC of healthy volunteer were incubated with another volunteer for 4 days. IL-17 and IL-10 concentrations in culture supernatants as determined by ELISA