The crush model of injury in skeletal muscle is widely used in the investigation of tissue degeneration and regeneration. Previously, such trauma has been induced by using forceps to crush the muscle, commonly applying sufficient pressure to bring the mid-arms of the forceps together. This report introduces a reliable electromechanical device designed to generate reproducible focal lesions in skeletal muscle of mice. The tibialis anterior was crushed in 17 young adult mice. Two days after injury, the muscles were examined microscopically. By morphometric analysis, it was determined that the volumes of the lesions produced were similar (mean 0.499 mm3±0.098, range 0.278−0.601 mm3), and that the full extent of the damaged muscle was easily distinguished and readily quantifiable. This will allow a more precise comparison in future investigations into regenerative differences between age groups, satellite cell activation and the inflammatory response.
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机译：骨骼肌损伤的挤压模型在组织变性和再生研究中被广泛使用。以前，这种创伤是通过使用镊子挤压肌肉而引起的，通常会施加足够的压力以将镊子的中臂拉到一起。该报告介绍了一种可靠的机电设备，旨在在小鼠骨骼肌中产生可再现的局灶性病变。胫骨前部被压在17只成年小鼠中。受伤两天后，用显微镜检查肌肉。通过形态计量学分析，确定所产生的病变的体积相似（平均为0.499 mm 3 ±0.098，范围为0.278−0.601 mm 3 ），并且受损肌肉的程度易于区分且易于量化。这将允许在将来的研究中对年龄组之间的再生差异，卫星细胞激活和炎症反应进行更精确的比较。
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