Distribution of mRNA for the GABA transporter GAT-1 in the rat brain: evidence that GABA uptake is not limited to presynaptic neurons.

摘要

Cells containing mRNA for the gamma-aminobutyric acid (GABA) transporter GAT-1 were identified in rat brain by in situ hybridisation. They were found in most of the known locations of GABAergic neurons, as defined by the distribution of mRNA for glutamic acid decarboxylase, the synthetic enzyme for GABA. Within the cerebellum there was substantial labelling of basket and stellate cells in the molecular layer, and of Golgi cells but no others in the granule cell layer. Many Purkinje cells were unlabelled while others, particularly in the hemispheres, were moderately labelled. Many of the Purkinje cells negative for GAT-1 mRNA had adjacent intensely labelled small cells whose size and position corresponded to Bergmann glia. Numerical comparison of cells labelling for GAT-1 mRNA and the mRNAs for the two known isoforms of glutamic acid decarboxylase were made on serial sections of cerebral cortex. Cells positive for GAT-1 mRNA were more numerous, indicating that expression of the transporter is not just limited to GABAergic cells and we suggest that it may also be expressed postsynaptically by some non-GABAergic neurons.