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Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis

机译:通过RFLP分析从叙利亚中部地区人类皮肤样本中分离出的利什曼原虫物种的特征

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摘要

Cutaneous leishmaniasis (CL) is an endemic disease and a public health problem in Hama governorate located in the central region of Syria. The aim of this study was to characterize Leishmania species isolated from human skin samples. A polymerase chain reaction, restriction fragment length polymorphism (PCR-RFLP) assay, was performed on skin lesion material samples from 32 patients with confirmed CL by direct microscopic examination in order to prove its usefulness and efficiency for identification of Leishmania species. Leishmania tropica (L. tropica) is confirmed as an etiologic agent of CL in this area.
机译:皮肤利什曼病(CL)是叙利亚中部哈马省的地方病和公共卫生问题。这项研究的目的是表征从人类皮肤样本中分离出的利什曼原虫物种。通过直接显微镜检查对32例确诊为CL的患者的皮肤病变材料样品进行了聚合酶链反应,限制性片段长度多态性(PCR-RFLP)分析,以证明其在鉴定利什曼原虫种类方面的效用和效率。热带利什曼原虫(L. tropica)被确认为该地区CL的病因。

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