Accumulation of intermediate denitrifying compounds inhibiting biological denitrification on cathode in Microbial Fuel Cell

摘要

BackgroundBio-cathode denitrifying microbial fuel cell (MFC) is a promising bio-electrochemical system (BES) where both the reactions of anodic oxidation and cathodic reduction are catalyzed by microorganisms. In this nitrogen removal process, a complete biological denitrification from nitrate (NO3^-) to molecular nitrogen (N2) was achieved by four reduction steps, forming nitrite (NO2⁻), nitric oxide (NO) and nitrous oxide (N2O) as intermediate compounds. These enzymatic catalysis reductions are often slowed down on cathode electrode at the higher cathodic nitrate loading. This study investigated the cause for inhibition of the biological denitrification in a three-chambered MFC where the middle chamber acted as denitrifying bio-cathode and the two chambers at the side acted as bio-anode. Carbon fiber brushes were used as electrodes and nafion membranes were used as separator between the chambers.