A method for purification of marble spleen disease virus (MSDV) from the spleens of infected turkeys and pheasants is described. It combines chloroform or fluorocarbon extraction with subsequent purification by centrifugation on a cushion of cesium chloride (CsCl). Further purification of MSDV was accomplished with a CsCl equilibrium density gradient. A viral buoyant density of approximately 1.32 to 1.33 g/cm3 was determined. Negative-stain electron microscopy revealed that virus isopycnically banded by CsCl gradient had non-enveloped icosahedral capsids composed of 252 capsomeres. The direct colorimetric diphenylamine assay indicated that MSDV has deoxyribonucleic acid as its nucleic acid. The above evidence demonstrates that MSDV is an avian adenovirus, the first recognized in the ring-necked pheasant (Phasianus colchicus L.).
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机译:描述了一种从受感染的火鸡和野鸡的脾中纯化大理石脾病病毒(MSDV)的方法。它将氯仿或碳氟化合物萃取与随后通过在氯化铯(CsCl)垫上离心分离而纯化相结合。 MSDV的进一步纯化通过CsCl平衡密度梯度完成。测定的病毒浮力密度约为1.32至1.33 g / cm3。负染色电子显微镜显示,通过CsCl梯度等渗带状病毒具有未被包裹的二十面体衣壳,该衣壳由252个衣壳体组成。直接比色二苯胺测定表明MSDV具有脱氧核糖核酸作为其核酸。以上证据表明,MSDV是一种禽腺病毒,最早在环颈野鸡(Phasianus colchicus L.)中发现。
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