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首页> 美国卫生研究院文献>Genome Research >Gene Survey of the Pathogenic Protozoan Trypanosoma cruzi
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Gene Survey of the Pathogenic Protozoan Trypanosoma cruzi

机译：致病性原生动物克氏锥虫的基因研究

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摘要

We have performed a survey of the active genes in the important human pathogen Trypanosoma cruzi by analyzing 5013 expressed sequence tags (ESTs) generated from a normalized epimastigote cDNA library. Clustering of all sequences resulted in 771 clusters, comprising 54% of the ESTs. In total, the ESTs corresponded to 3054 transcripts that might represent one-fourth of the total gene repertoire in T. cruzi. About 33% of the T. cruzi transcripts showed similarity to sequences in the public databases, and a large number of hitherto undiscovered genes predicted to be involved in transcription, cell cycle control, cell division, signal transduction, secretion, and metabolism were identified. More than 140 full-length gene sequences were derived from the ESTs. Comparisons with all open reading frames in yeast and in Caenorhabditis elegans showed that only 12% of the T. cruzi transcripts were shared among diverse eukaryotic organisms. Comparison with other kinetoplastid sequences identified 237 orthologous genes that are shared between these evolutionarily divergent organisms. The generated data are a useful resource for further studies of the biology of the parasite and for development of new means to combat Chagas' disease.[The sequence data described in this paper have been submitted to the dbEST database under nos. TENU0001–TENU5214 and the following: -, -, -, -, -, AA87464-, -, -, -{"type":"entrez-nucleotide","attrs":{"text":"AA897341","term_id":"3033961"}}AA897341, {"type":"entrez-nucleotide","attrs":{"text":"AA879376","term_id":"2988341"}}AA879376-{"type":"entrez-nucleotide","attrs":{"text":"AA879401","term_id":"2988511"}}AA879401, {"type":"entrez-nucleotide","attrs":{"text":"AA882494","term_id":"2991605"}}AA882494-{"type":"entrez-nucleotide","attrs":{"text":"AA882518","term_id":"2991629"}}AA882518, {"type":"entrez-nucleotide","attrs":{"text":"AA883036","term_id":"2992635"}}AA883036-{"type":"entrez-nucleotide","attrs":{"text":"AA883051","term_id":"2992650"}}AA883051, {"type":"entrez-nucleotide","attrs":{"text":"AI005678","term_id":"3215188"}}AI005678-{"type":"entrez-nucleotide","attrs":{"text":"AI005729","term_id":"3215239"}}AI005729, {"type":"entrez-nucleotide","attrs":{"text":"AI007342","term_id":"3216852"}}AI007342-{"type":"entrez-nucleotide","attrs":{"text":"AI007441","term_id":"3216951"}}AI007441, {"type":"entrez-nucleotide","attrs":{"text":"AI021797","term_id":"3237410"}}AI021797-{"type":"entrez-nucleotide","attrs":{"text":"AI021884","term_id":"3237497"}}AI021884, {"type":"entrez-nucleotide","attrs":{"text":"AI026370","term_id":"3244196"}}AI026370-{"type":"entrez-nucleotide","attrs":{"text":"AI026615","term_id":"3244441"}}AI026615, AI037797-AI037846, AI043247-AI043343, AI043427-AI043502, AI046026-AI046290, AI050095-AI050219, AI053146-AI053397, AI057644-AI057957, AI065169-AI065425, AI066117-AI066391, AI069556-AI069908, AI073286-AI073332, AI075466-AI075620, AI077051-AI077281, AI078888-AI079000, AI080790-AI080916, AI083097-AI083245, AI110290-AI110405, AI110412-AI110512, AW324789-AW325325, AW329885-AW330435, and AW621062-AW621094. The sequences are also available at www.genpat.uu.se/tryp/tryp.html.]
机译：我们已经对重要的人类病原体克氏锥虫进行了活性基因的调查，方法是分析从标准化的附鞭毛虫cDNA文库生成的5013个表达的序列标签（EST）。所有序列的聚类产生771个聚类，占EST的54％。总体而言，EST对应于3054个转录本，可能占克鲁维酵母总基因谱的四分之一。大约33％的克鲁维氏T转录本显示与公共数据库中的序列相似，并且鉴定了迄今尚未发现的大量预计与转录，细胞周期控制，细胞分裂，信号转导，分泌和代谢有关的基因。来自EST的超过140个全长基因序列。与酵母和秀丽隐杆线虫中所有开放阅读框的比较表明，在不同的真核生物之间仅共享克鲁维氏T转录本的12％。与其他动素体序列的比较确定了237个直系同源基因，这些基因在这些进化分化的生物之间共享。产生的数据为进一步研究该寄生虫的生物学和开发对抗南美锥虫病的新手段提供了有用的资源。[本文描述的序列数据已提交至dbEST数据库，编号为。 TENU0001–TENU5214和以下版本：-，-，-，-，-，AA87464-，-，-，- {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AA897341“，” term_id“：” 3033961“}} AA897341 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AA879376”，“ term_id”： “ 2988341”}} AA879376 - {“ type”：“ entrez-nucleotide” ，“ attrs”：{“ text”：“ AA879401”，“ term_id”：“ 2988511”}} AA879401 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AA882494”，“ term_id”：“ 2991605”}} AA882494 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AA882518”， “ term_id”：“ 2991629”}} AA882518 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AA883036“，” term_id “：”“ 2992635”}} AA883036 - {“ type”：“ entrez-核苷酸“，” attrs“：{” text“：” AA883051“，” term_id“：” 2992650“}} AA883051 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI005678“，” term_id“：” 3215188“}} AI005678 -< id =“ __ tag_777644262” class =“ tag_hotlink” href =“ / nuccore / AI005729” ref =“ / nuccore / AI005729”> {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI005729 “，” term_id“：” 3215239“}} AI005729 ， {” type“ ：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI007342”，“ term_id”：“ 3216852”}} AI007342 - {” type“：” entr ez-nucleotide“，” attrs“：{” text“：” AI007441“，” term_id“：” 3216951“}} AI007441 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI021797“，” term_id“：” 3237410“}} AI021797 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”： “ AI021884”，“ term_id”：“ 3237497”}} AI021884 ， {“ type“：” entrez-nucleotide“，” attrs“：{” text“：” AI026370“，” term_id“：” 3244196“}} AI026370 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI026615”，“ term_id”：“ 3244441”}} AI026615 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI037797”，“ term_id“：” 3269271“}} AI037797 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI037846”，“ term_id”：“ 3269270”}}} AI037846 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI043247“，“ term_id”：“ 3286504”}} AI043247 - < span class =“ tag-json” style =“ display：none”> {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI043343”，“ term_id”：“ 3286600”}}} < / span> AI043343 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI043427“，” term_id“：” 3290210“}} AI043427 - {“ type”：“ entrez-nucleotide“，” attrs“：{” text“：” AI043502“，” term_id“：” 3290285“}} AI043502 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text“：” AI046026“，” term_id“：” 3293924“}} AI046026 - {“ type”：“ entrez-nucleotide”， “ attrs”：{“ text”：“ AI046290”，“ term_id”：“ 3293923”}} AI046290 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI050095 “，” term_id“：” 3299212“}} AI050095 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI050219”，“ term_id”：“ 3299336”}} AI050219 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI053146”，“ term_id”：“ 3321025”}} AI053146 -<一个id =“ __ tag_777644223” class =“ tag_hotlink” href =“ / nuccore / AI053397” ref =“ / nuccore / AI05339 7“> {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI053397“，” term_id“：” 3321024 “}} AI053397 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI057644“，” term_id“：” 3331510“}}} AI057644 - {“ type“：” entrez-nucleotide“，” attrs“：{” text“：” AI057957“，” term_id“：” 3331823“}} AI057957 ， {“ type”：“ entrez-nucleotide”，“ attrs “：{” text“：” AI065169“，” term_id“：” 3340576“}} AI065169 - {“ type”：“ entrez-nuc leotide“，” attrs“：{” text“：” AI065425“，” term_id“：” 3340832“}} AI065425 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“ ：“ AI066117”，“ term_id”：“ 3366282”}} AI066117 - {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI066391“，” term_id“：” 3366281 “}} AI066391 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI069556“，” term_id“：” 3392531“}}} AI069556 - {“类型“：” entrez-nucleotide“，” attrs“：{” text“：” AI069908“，” term_id“：” 3392883“}}} AI 069908 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI073286“，” term_id“：” 3399480“}} AI073286 - {” type“：” entrez-nucleotide “，” attrs“：{” text“：” AI073332“，” term_id“：” 3399526“}} AI073332 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“： “ AI075466”，“ term_id”：“ 3404587”}} AI075466 - {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI075620“，” term_id“：” 3404571“ }} AI075620 ， {“ type “：” entrez-nucleotide“，” attrs“：{” text“：” AI077051“，” term_id“：” 3411359“}} AI077051 - {” type“：” entrez-nucleotide“，” attrs“ ：{“ text”：“ AI077281”，“ term_id”：“ 3411358”}} AI077281 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI078888”，“ term_id“：” 3415235“}} AI078888 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI079000”，“ term_id”：“ 3415176”}}} AI079000 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI080790 “，” term_id“：” 3417042“}} AI080790 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI080916”，“ term_id”：“ 3417168”}} AI080916 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AI083097”，“ term_id”：“ 3421520”}}} AI083097 -< id =“ __ tag_777644225” class =“ tag_hotlink” href =“ / nuccore / AI083245” ref =“ / nuccore / AI083245”> {“ type”： “ entrez-nucleotide”，“ attrs”：{“ text”：“ AI083245”，“ term_id”：“ 3421668”}} AI083245 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI110290“，” term_id“：” 3482826“}} AI110290 - {“ type”：“ entrez -nucleotide“，” attrs“：{” text“：” AI110405“，” term_id“：” 3482825“}} AI110405 ， {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text “：” AI110412“，” term_id“：” 3492619“}} AI110412 - {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AI110512“，” term_id“：” 3492566“}} AI110512 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AW324789“，” ter m_id“：” 6760710“}} AW324789 - {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ AW325325”，“ term_id”：“ 6761246”}}} AW325325 ， {” type“：” entrez-nucleotide“，” attrs“：{” text“：” AW329885“，” term_id“：” 6807004“}} AW329885 - {“ type”：“ entrez-核苷酸”，“ attrs”：{“ text”：“ AW330435”，“ term_id”：“ 6807493”}} AW330435 和 {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text “：” AW621062“，” term_id“：” 7327651“}} AW621062 - {“ type”：“ entrez-nucleotide”， “ attrs”：{“ text”：“ AW621094”，“ term_id”：“ 7327683”}} AW621094 。也可以在 www.genpat.uu.se/tryp/tryp.html。]

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