Molecular characterization and serodiagnostic potential of

摘要

The amino acid sequence alignment analysis of Echinococcus granulosus hexokinase (EgHK) with the homologous hexokinase (HK) from other parasite species: Echinococcus granulosus (E. g, MW292450), Echinococcus multiloculari (E. m, {"type":"entrez-protein","attrs":{"text":"CDS38325.1","term_id":"674576035"}}CDS38325.1), Hymenolepis microstomia (H. m, {"type":"entrez-protein","attrs":{"text":"CDS34381.1","term_id":"674586534"}}CDS34381.1 ), Clonorchis sinensis (C. s, {"type":"entrez-protein","attrs":{"text":"GAA52956.1","term_id":"358253389"}}GAA52956.1), Schistosoma mansoni (S. m, {"type":"entrez-protein","attrs":{"text":"AAA29894.2","term_id":"11387389"}}AAA29894.2), Schistosoma japonicum (S. j, {"type":"entrez-protein","attrs":{"text":"CAX74187.1","term_id":"226484556"}}CAX74187.1), Trypanosoma cruzi (T. c, {"type":"entrez-protein","attrs":{"text":"AAL93565.1","term_id":"19703093"}}AAL93565.1), Trypanosoma brucei (T. b, {"type":"entrez-protein","attrs":{"text":"CAC69958.1","term_id":"15626363"}}CAC69958.1), Toxoplasma gondii (T. g, {"type":"entrez-protein","attrs":{"text":"BAB55664.1","term_id":"14164312"}}BAB55664.1), Plasmodium falciparum (P. f, {"type":"entrez-protein","attrs":{"text":"ETW53097.1","term_id":"574991979"}}ETW53097.1), Mus musculus (M. m, {"type":"entrez-protein","attrs":{"text":"AAB57759.1","term_id":"1850140"}}AAB57759.1), Homo sapiens HK-1 (H. s HK-1, NP000179.2), Homo sapiens HK-2 (H. s HK-2, NP000180.2), Homo sapiens HK-3 (H. s HK-3, NP002106.2), Homo sapiens HK-4 (H. s HK-4, {"type":"entrez-protein","attrs":{"text":"AAH01890.1","term_id":"12804883"}}AAH01890.1). In the amino acid sequence, the conserved domains for glucose, glucose 6-phosphate (G-6-P) and adenosine triphosphate (ATP) are illustrated in red, blue and green rectangles, respectively. The letters marked with pink underlines are putative glucose binding sites (144aa, 145aa, 146aa, 161aa, 162aa, 196aa, 197aa, 217aa, 221aa, 222aa, 223aa, 249aa, 280aa, 283aa). Nine potential B-cell epitopes (ep1:62–72 aa; ep2:93–102 aa; ep3:111–115 aa; ep4:186–191 aa; ep5:248–256 aa; ep6:289–296 aa; ep7:343–352 aa; ep8:370–375 aa; ep9:448–456 aa) are marked with orange overlines