机译
质子泵抑制剂酸泵拮抗剂或吲哚美辛磷脂酰胆碱对吲哚美辛诱导的小肠损伤的体外和体内保护
摘要:Background/AimsProton pump inhibitors (PPI) are widely used to prevent NSAID-induced peptic ulcers. NSAIDs produce small intestinal injury and some PPIs have been reported to have a protect against NSAID-induced small bowel injury in rats. To compare PPIs, revaprazan, and phosphatidylcholine-associated indomethacin (Indo-PC) for protection against indomethacin (Indo) induced small bowel injury. Methods: Rat intestinal epithelial cells (IEC-6) were pretreated with omeprazole, lansoprazole or revaprazan prior to exposure to Indo or Indo-PC. Cell viability was assessed by methyl thiazolyl tetrazolium assay. Omeprazole, lansoprazole or revaprazan were administered orally to rats prior to vehicle or Indo. Indo-PC was administered alone. After 24h small intestinal erosions were counted; intestinal bleeding was assessed as hemoglobin concentration of small intestinal fluid. Results: Omeprazole, lansoprazole and revaprazan did not protect against Indo-induced IEC-6 cell injury. Indo-PC was less damaging in vitro than Indo alone. In vivo neither omeprazole nor lansoprazole protected against Indo-induced small bowel injury, however, revaprazan pretreatment and Indo-PC resulted in significantly fewer erosions (>50% reduction) or bleeding (>80% reduction).