首页> 美国卫生研究院文献>International Journal of Nanomedicine >Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
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Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody

机译:使用与抗VEGFR2 / KDR抗体偶联的免疫脂质体向肝血管瘤内皮细胞递送腹水酸钠

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摘要

Hemangioma is a common benign tumor affecting infants. In this study, we prepared sodium morrhuate immunoliposomes through encapsulation of sodium morrhuate with liposomes coupled with an anti-VEGFR2/KDR antibody and examined its effect on the biology of human hemangioma endothelial cells (HECs). It was found that compared to the liposomal sodium morrhuate group, treatment with sodium morrhuate immunoliposomes facilitated cell detachment and apoptotic death. Confocal microscopy analysis revealed that sodium morrhuate immunoliposomes had a higher binding activity to HECs than liposomal sodium morrhuate. Apoptosis analysis further demonstrated that treatment with liposomal sodium morrhuate or sodium morrhuate immunoliposomes significantly induced apoptosis in HECs, compared to the control group. Western blot analysis revealed an induction of caspase-3 and caspase-9 levels and reduction of caspase-8 and Bcl-2 levels in HECs treated with liposomal sodium morrhuate or sodium morrhuate immunoliposomes. Taken together, these results indicate that sodium morrhuate immunoliposomes have an increased capacity to target HECs and promote mitochondrial apoptosis. Therefore, sodium morrhuate immunoliposomes may represent a promising agent in the treatment of hemangiomas.
机译:血管瘤是影响婴儿的常见良性肿瘤。在这项研究中,我们通过将脂质体与抗VEGFR2 / KDR抗体偶联的脂质体包囊法制备了脂质体钠免疫脂质体,并研究了其对人血管瘤内皮细胞(HECs)生物学的影响。已经发现,与脂质体肝硬化钠组相比,肝硬化钠免疫脂质体的治疗促进了细胞的脱离和凋亡的死亡。共聚焦显微镜分析表明,与脂质体次酸钠相比,次戊酸钠免疫脂质体对HEC的结合活性更高。凋亡分析进一步证明,与对照组相比,用脂质体肝硬化钠或肝硬化钠免疫脂质体治疗可显着诱导HEC凋亡。 Western blot分析显示,用脂质体肝硬化钠或肝硬化钠免疫脂质体治疗的HEC中caspase-3和caspase-9的水平被诱导,caspase-8和Bcl-2的水平降低。综上所述,这些结果表明,肝硬化的免疫脂质体钠具有更高的靶向HECs和促进线粒体凋亡的能力。因此,腹腔注射免疫脂质体钠可能代表血管瘤治疗中有希望的药物。

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