首页> 美国卫生研究院文献>Iranian Red Crescent Medical Journal >Effects of Acellular Amniotic Membrane Matrix and Bone Marrow-Derived Mesenchymal Stem Cells in Improving Random Skin Flap Survival in Rats
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Effects of Acellular Amniotic Membrane Matrix and Bone Marrow-Derived Mesenchymal Stem Cells in Improving Random Skin Flap Survival in Rats

机译:脱细胞羊膜基质和骨髓源性间充质干细胞在改善大鼠随机皮瓣存活中的作用

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摘要

BackgroundThe necrotic skin flap represents a great challenge in plastic and reconstructive surgery. In this study, we evaluated the effect of bioscaffolds, acellular amniotic membranes (AAMs), and bone marrow-derived mesenchymal stem cells (BM-MSCs) on random skin flap (RSF) survival in rats by applying a cell-free extracellular matrix scaffold as a supportive component for the growth and proliferation of BM-MSCs on RSFs. AAM matrix scaffolds were created by incubating AMs in ethylenediaminetetraacetic acid 0.05% at 37°C, and cell scrapers were used.
机译:背景坏死性皮瓣代表了整形和重建手术中的巨大挑战。在这项研究中,我们通过应用无细胞的细胞外基质支架,评估了生物支架,脱细胞羊膜(AAM)和骨髓源性间充质干细胞(BM-MSC)对大鼠随机皮瓣(RSF)存活的影响。作为BM-MSC在RSF上生长和增殖的支持成分。通过将AM在0.05%的乙二胺四乙酸中于37°C孵育来创建AAM基质支架,并使用细胞刮刀。

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