Electroejaculated Baboon (Papio anubis) Sperm Requires a Higher Dosage of Pentoxifylline to Enhance Motility

摘要

>Purpose: Sperm collected by electroejaculation often showpoor motility. The objective was to determine whether theaddition of the phosphodiesterase inhibitor, pentoxifylline,would stimulate electroejaculated baboon sperm motility.>Methods: Electroejaculation was performed on severaloccasions on a male baboon and sperm collected afterfamiliarization. Pentoxifylline was tested at the standardconcentration (1 mg/ml) and at twice the concentration. Spermparameters were evaluated using a sperm motility analyzer,as well as acrosome and DNA integrity techniques.>Results: Sperm exposed to 2 mg/ml pentoxifylline had highertotal motility when compared with the control and 1 mg/mltreatment. Rapid progression and velocities were higher afterpentoxifylline. The acridine orange DNA normality testshowed that over 90;pc of collected sperm had intactunfragmented DNA. About half the sperm population had normalmorphology and intact acrosomes. A low percentage hadcytoplasmic droplets.>Conclusions: Sperm collected by rectal probeelectroejaculation required a higher concentration (2 mg/ml) ofpentoxifylline for enhanced total motility, rapid progression, andhigher velocity. This suggested differences in membraneproperties or phosphodisterase activity in electrojeaculatedsperm. The electroejaculation procedure did not denaturesperm DNA at the acridine orange assay level nor were theacrosomes disrupted. The present study also documentedunique information on baboon kinematic parameters.