Isolation and Purification of an Early Pregnancy Factor–like Molecule from Culture Supernatants Obtained from Lymphocytes of Pregnant Women: II. Identification of the Molecule as a FC-Receptor–like Molecule: A Preliminary Report

摘要

>Purpose:Early pregnancy factor (EPF)–like activity from culture supernatants obtained from stimulated lymphocytes of pregnant women was characterized and identified.>Methods:The enzyme-linked immunosorbent assay depending on the presence of “Fc” receptors on bovine spermatozoa was used to identify the EPF-like molecule purified by gel filtration and reverse-phase high-performance liquid chromatography.>Results:The results indicated that the crude lymphocyte culture supernatant, the EPF-positive G IV fraction obtained on gel filtration, and the EPF-positive reverse-phase high-performance liquid chromatography protein readily bound with the different concentrations of aggregated human γ-globulin in a manner similar to that in which the standard control of aggregated human γ-globulin binds to the bovine spermatozoa.>Conclusions:EPF-like activity synthesized and secreted by lymphocytes during pregnancy may be a Fc-receptor–like molecule.