Centrifugal Pulsing Increases the Recovery of Viable Spermatozoa

摘要

>Purpose:Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation.>Methods:A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5°C in TEST-yolk results determined the efficacy of the centrifugation techniques.>Results:The number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5°C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standard centrifugation.>Conclusions:Pulsing the relative centrifugal field during centrifugation yields a higher number of viable spermatozoa.