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Surface Layers of Clostridium difficile Endospores

机译:艰难梭菌内生孢子的表面层

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摘要

Clostridium difficile is an important human pathogen and one where the primary cause of disease is due to the transmission of spores. We have investigated the proteins found in the outer coat layers of C. difficile spores of pathogenic strain 630 (CD630). Five coat proteins, CotA, CotB, CotCB, CotD, and CotE, were shown to be expressed on the outer coat layers of the spore. We demonstrate that purified spores carry catalase, peroxiredoxin, and chitinase activity and that this activity correlates with the predicted functions of three spore coat proteins identified here, CotCB, CotD, and CotE. CotCB and CotD are putative manganese catalases, and CotE is a novel bifunctional protein with peroxiredoxin activity at its amino terminus and chitinase activity at its carboxy terminus. These enzymes could play an important role in coat assembly by polymerizing protein monomers in the coat. CotE, in addition to a role in macromolecular degradation, could play an important role in inflammation, and this may be of direct relevance to the development of the gastrointestinal symptoms that accompany C. difficile infection. Although specific enzyme activity has not yet been assigned to the proteins identified here, this work provides the first detailed study of the C. difficile spore coat.
机译:艰难梭菌是一种重要的人类病原体,也是主要的病因是孢子传播的病原体。我们已经研究了在致病性菌株630(CD630)的艰难梭菌孢子外层中发现的蛋白质。五个外壳蛋白,CotA,CotB,CotCB,CotD和CotE,在孢子的外涂层上表达。我们证明纯化的孢子携带过氧化氢酶,过氧化物酶和几丁质酶活性,并且该活性与此处鉴定的三种孢子外壳蛋白,CotCB,CotD和CotE的预测功能相关。 CotCB和CotD是推定的锰过氧化氢酶,CotE是一种新型双功能蛋白,在其氨基末端具有过氧化物酶活性,在其羧基末端具有几丁质酶活性。这些酶可以通过在外套中聚合蛋白质单体在外套组装中发挥重要作用。除了在大分子降解中起作用外,CotE可能在炎症中起重要作用,这可能与艰难梭菌感染所伴随的胃肠道症状的发生直接相关。尽管尚未将特定的酶活性分配给此处鉴定的蛋白质,但这项工作提供了艰难梭菌孢子皮的首次详细研究。

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