A microfluidic channel made entirely out of polyethylene glycol (PEG), not PEG coating to silicon or polydimethylsiloxane (PDMS) surface, was fabricated and tested for its reusability in particle immunoassays and passive protein fouling, at relatively high target concentrations (1 mg ml-1). The PEG devices were reusable up to ten times while the oxygen-plasma-treated polydimethyl siloxane (PDMS) device could be reused up to four times and plain PDMS were not reusable. Liquid was delivered spontaneously via capillary action and complicated bonding procedure was not necessary. The contact angle analysis revealed that the water contact angle on microchannel surface should be lower than ~60°, which are comparable to those on dried protein films, to be reusable for particle immunoassays and passive protein fouling.
展开▼
机译:制备了完全由聚乙二醇(PEG)制成的微流体通道,而不是在硅或聚二甲基硅氧烷(PDMS)表面上形成PEG涂层,并在相对较高的目标浓度(1 mg ml < sup> -1 sup>)。 PEG设备最多可重复使用十次,而经氧等离子体处理的聚二甲基硅氧烷(PDMS)设备最多可重复使用四次,而普通PDMS无法重复使用。液体通过毛细作用自发地输送,不需要复杂的结合程序。接触角分析表明,微通道表面的水接触角应小于〜60°,与干蛋白膜上的水相近,以便可用于颗粒免疫分析和被动蛋白结垢。
展开▼