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Introduction of customized inserts for streamlined assembly and optimization of BioBrick synthetic genetic circuits

机译:引入定制插入物以简化组装和优化BioBrick合成遗传电路

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摘要

BackgroundBioBrick standard biological parts are designed to make biological systems easier to engineer (e.g. assemble, manipulate, and modify). There are over 5,000 parts available in the Registry of Standard Biological Parts that can be easily assembled into genetic circuits using a standard assembly technique. The standardization of the assembly technique has allowed for wide distribution to a large number of users -- the parts are reusable and interchangeable during the assembly process. The standard assembly process, however, has some limitations. In particular it does not allow for modification of already assembled biological circuits, addition of protein tags to pre-existing BioBrick parts, or addition of non-BioBrick parts to assemblies.
机译:背景技术BioBrick标准生物零件旨在使生物系统更易于工程设计(例如组装,操纵和修改)。标准生物零件注册处提供了5,000多个零件,可以使用标准组装技术轻松将其组装到遗传回路中。组装技术的标准化已允许向大量用户广泛分发-零件在组装过程中可重复使用和互换。但是,标准组装过程有一些限制。特别是,它不允许修改已组装的生物回路,向预先存在的BioBrick零件添加蛋白质标签,或向装配体添加非BioBrick零件。

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