首页> 美国卫生研究院文献>Journal of Biomolecular Techniques : JBT >(w6) Current State and Future of Capillary Electrophoresis and Sanger Sequencing
【2h】

(w6) Current State and Future of Capillary Electrophoresis and Sanger Sequencing

机译:(w6)毛细管电泳和Sanger测序的现状和未来

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Capillary Electrophoresis remains a widely used, often irreplaceable, sequencing technology continuing to experience increasing demands. We believe that the speed, simplicity and high quality of reads for low volume sequencing needs will maintain the viability of this technology for many years to come. Although the main thrust of development in the sequencing space is now in NGS area, there are still steps which can be improved or adjusted as other methods mature. In this session we propose to cover: The effect of long-term storage of DNA under different conditions (temp/buffers) on sequencing, spectral characteristics, integrity and transformation efficiency; A Description of the Apollo system; and the Future of Capillary Electrophoresis and Sanger sequencing. In addition to the featured talks, there will be a question & answer component in which participants are encouraged to bring up any issues relevant to this topic.
机译:毛细管电泳仍然是一种广泛使用的,常常是不可替代的测序技术,其需求不断增长。我们相信,满足小批量测序需求的读取速度,简便性和高质量将在未来很多年内保持该技术的可行性。尽管测序领域的主要发展方向现在是在NGS领域,但随着其他方法的成熟,仍有一些步骤可以改进或调整。在本次会议中,我们建议讨论以下内容:在不同条件(温度/缓冲液)下长期保存DNA对测序,光谱特征,完整性和转化效率的影响;阿波罗系统的描述;以及毛细管电泳和Sanger测序的未来。除特色演讲外,还将有一个问答部分,鼓励参与者提出与该主题相关的任何问题。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号