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ISOLATION AND PRELIMINARY CHARACTERIZATION OF BACTERIOPHAGES FOR BACILLUS SUBTILIS

机译:芽孢杆菌的噬菌体的分离和初步鉴定

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摘要

Romig, W. R. (University of California, Los Angeles), and A. M. Brodetsky. Isolation and preliminary characterization of bacteriophages for Bacillus subtilis. J. Bacteriol. >82:135–141. 1961.—A simplified procedure for direct isolation of phages for Bacillus subtilis from soil was developed. Phage enrichment was accomplished by growing streptomycin-resistant B. subtilis in medium previously inoculated with the soil sample. Contaminating soil bacteria were eliminated by adding bactericidal quantities of streptomycin and the phages were isolated by conventional agar layer techniques. By this method 1 or more subtilis phages were isolated from 15 of 18 soil samples tested. Several of these phages were unusually sensitive to chloroform and all of them were relatively unstable when stored at refrigerator temperatures. Of 6 phages retained for study, 1 was temperate for B. subtilis, but attempts to obtain stably lysogenic bacteria following infection with this phage were unsuccessful. All 6 phages had identical host ranges and were able to lyse all strains of B. subtilis tested, as well as several related species of Bacillus.
机译:Romig,W. R.(加州大学洛杉矶分校)和A. M. Brodetsky。枯草芽孢杆菌噬菌体的分离和初步鉴定。 J.细菌。 > 82: 135-141。 1961年。开发了一种从土壤中直接分离枯草芽孢杆菌噬菌体的简化程序。噬菌体富集是通过在先前接种过土壤样品的培养基中培养抗链霉素的枯草芽孢杆菌来实现的。加入杀菌量的链霉素可消除污染的土壤细菌,并通过常规琼脂层技术分离噬菌体。通过这种方法,从18个被测土壤样品中的15个分离出1个或更多枯草噬菌体。这些噬菌体中有几个对氯仿异常敏感,并且在冰箱温度下保存时,它们都相对不稳定。在留作研究的6个噬菌体中,有1个是枯草芽孢杆菌的温带菌,但尝试获得这种噬菌体感染后获得稳定的溶菌细菌的尝试并未成功。所有6个噬菌体具有相同的宿主范围,并且能够裂解所有测试的枯草芽孢杆菌菌株以及几种相关的芽孢杆菌。

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