A273 THE CROHN’S DISEASE-ASSOCIATED PATHOBIONT ADHERENT-INVASIVE E. COLI (AIEC) INDUCES MITOCHONDRIAL FISSION IN EPITHELIAL CELLS IN ADVANCE OF APOPTOSIS

摘要

BackgroundAdherent-invasive E. coli are a putative etiological agent in a cohort of patients with Crohn’s disease. Mitochondrial dysfunction has been described in inflammatory bowel disease. Mitochondria are not distinct organelles; they exist as a dynamic network that constantly remodels via the processes of fission and fusion to meet the cells energy demands and allow recycling of damaged mitochondria. This is emerging as an area of interest in host-bacterial interaction but it is unknown if (and then how) infection with AIEC affects mitochondrial dynamics in epithelia and the consequences of this for the cell. >Hypothesis: AIEC induce mitochondrial fragmentation in intestinal epithelial cells in an invasion-dependent manner that requires ROS, resulting in apoptosis and decreased epithelial barrier function.