The regulation of ferroptosis by MESH1 through the activation of the integrative stress response

摘要

A Heatmap of the transcriptional difference between control or two independent MESH1 siRNA knockdown in RCC4 cells. MESH1 was silenced by siMESH1#1 or siMESH1#2 and profiled by RNA-seq. Color scale indicates log2-fold-change. B Top ten repressed Gene Ontology processes of the transcriptome of MESH1-silenced RCC4 cells. C, D GSEA analysis showed the enrichment of DNA replication geneset (C) and constituent of ribosome (D) in the MESH1 knockdown cells. E–H mRNA abundance of MESH1 (E), CDK2 (F), E2F1 (G), and RRM2 (H) as validated by rt-qPCR. mRNA abundance was determined by rt-qPCR, normalized by β-actin, and presented in relative ratio to siNT (non-targeting) treatment. n = 3 biological replicates. Statistical analysis: ANOVA with Tukey HSD post hoc test, **P < 0.01.