Induction of immune response after SARS-CoV-2 mRNA BNT162b2 vaccination in healthcare workers

摘要

SARS-CoV-2 antibody responses (Panels A-B). Anti-RBD and anti-trimeric S titers assessed by Elecsys® Anti-SARS-CoV-2 S assay (Roche, cut-off 0.8 U/mL) (Panel A) LIAISON® SARS-CoV-2 TrimericS IgG assay (Diasorin, cut-off 13 AU/mL) (Panel B) before vaccine administration (Day 0), at 7 (Day 7), 21 (Day 21) and 28 (Day 28) days after the first dose, against participants' age. Anti-S titers at different time-points were compared according to age by the Mann-Whitney test. Scatter dot plots denote interquartile ranges and horizontal bars median end-point titers. Whisker end-points denote the maximum and minimum values below or above the median at 1.5 times the interquartile range. The box indicates the width of titers' distribution proportionate to the number of points at that Y value. Blue and red dots defined 18–55 and ≥ 56 years ranges, respectively. Each dot represents a single patient. Frequency of trimeric spike-specific B and T cells 7 days after the first vaccine dose (Panels C, D, E). The frequency of trimeric spike-specific B cells before and 7 days after the first dose was showed by violin plot (Panel C). Connected scatter plot depicts the percentage in ELISpot of trimeric spike-specific IgM memory B cells (calculated within the total IgM memory B cells) (Panel D). Antigen-specific CD4+T cells expansion at 7 days following the first dose of vaccination is shown by violin plot (Panel E). Blue and red dots defined values at day 0 and day 7, respectively. Each dot represents a single patient. Statistical significance was calculated using the paired-Wilcoxon test and a P-value <0.05 was considered significant. Ag = antigen. Number of COVID-19 cases among HCWs and number of vaccinations against weeks (Panel F). Black line indicates the number of COVID-19 among HCWs. Red bars indicate the number of second doses against date. Pink bars indicate the number of first doses against date. SARS-CoV-2 infections occurring among HCWs after the first vaccine dose against days after the first vaccine dose (Panel G). Black bars indicate the number of infection cases. No infection occurred after day 14. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)