Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration

摘要

A Transfection of Schwann cells with LIF-siRNA-1 or LIF-siRNA-2 reduces the mRNA abundance of LIF. B LIF-siRNA-1 or LIF-siRNA-2 transfection increases Schwann cell proliferation. Red color indicates EdU-positive cells and blue color indicates nucleus. Scale bar indicates 100 μm. C LIF-siRNA-1 or LIF-siRNA-2 transfection increases Schwann cell migration. Violet color indicates migrated cells. Scale bar indicates 50 μm. D LIF-siRNA-1 or LIF-siRNA-2 transfection decreases cleaned areas between scratches. Scale bar indicates 100 μm. E Transfection of Schwann cells with LIF-overexpressing lentivirus (LIF-Lv) reduces the mRNA abundance of LIF. F LIF-overexpressing lentivirus transfection decreases Schwann cell proliferation. Red color indicates EdU-positive cells and blue color indicated nucleus. Scale bar indicates 100 μm. G LIF-overexpressing lentivirus transfection decreases Schwann cell migration. Violet color indicates migrated cells. Scale bar indicates 50 μm. H LIF-overexpressing lentivirus transfection increases cleaned areas between scratches. Scale bar indicates 100 μm. Data were presented as means ± SEM. *p value <0.05 versus control-siRNA (Con-siRNA) or control-lentivirus (Con-Lv).