PIWIL2 interacting with IKK to regulate autophagy and apoptosis in esophageal squamous cell carcinoma

摘要

Total mRNAs and proteins were extracted from tissue samples of eight individual ESCC patients, and subjected to RT-PCR (a) and western blot (b) respectively. T, tumor tissues; P, paratumor tissues. c ESCC cell lines (KYSE150, KYSE180, KYSE510, and Eca-109) and normal esophageal epithelial cell line Het-1A were subjected to expression analysis of PIWIL2 using western blotting (WB). d Kaplan–Meier curve depicting the long-term survival of the ESCC patients (n = 109). The curves were stratified based on the PIWIL2 level scored by intensity (0–3) and area (0–4) of the staining with TMA and IH technology (Log-rank test, p = 0.005). e Patients were randomly selected from PIWIL2 high-expression group and low-expression group (two from each). Biomarkers of autophagy (LC3 and P62) were analyzed using immunohistochemistry. Scale bar, 20 µm. *p < 0.05; ***p < 0.001.