Maintaining Inducibility of Dermal Follicle Cells on Silk Fibroin/Sodium Alginate Scaffold for Enhanced Hair Follicle Regeneration

摘要

Patients with severe skin damage need to transplant artificial skin substitutes to achieve repair, and often the repaired part cannot achieve the regeneration of accessory organs such as hair follicles. It is very important to study artificial skin substitutes that can promote the regeneration of hair follicles. Dermal papilla cells in hair follicles can express a variety of growth factors, which play an important role in the growth of hair follicles. Dermal papilla cells were used as seed cells and cultured with a biomimetic silk fibroin/sodium alginate scaffold similar to the extracellular matrix around the dermal papilla to explore cell-compatibility on the scaffold. Our research found that a silk fibroin/sodium alginate scaffold is a good biomimetic of the extracellular matrix structure, and that the dermal papilla cells could maintain normal morphology and aggregate growth characteristics on the scaffold. We then proved through animal experiments that this scaffold can promote skin repair and induce hair follicle regeneration.