Involvement of the endocannabinoid system in the inhibition of Sindbis virus replication: a preliminary study

摘要

a Validation CB1 receptor expression in human liver (Huh7) cell line. Cells were exposed to a primary antibody (1Ab) that binds specifically to the cannabinoid receptor I (ab23703). The antibody is conjugated with a fluorophore for detection and quantification. Cells with no 1Ab, did not give off any signal, whereas cells exposed to the primary antibody-targeting CB1 resulted in high level of detection, indicating a strong presence of the CB1 receptor. b Human liver (Huh7) cells can successfully be infected with Sindbis virus. After 24 h, nearly 10% of cells are infected with Sindbis virus